| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.045814 |
| Chromosome: | chromosome 3 |
| Location: | 4123454 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g173300 | (1 of 6) IPR007577//IPR029044 - Glycosyltransferase, DXD sugar-binding motif // Nucleotide-diphospho-sugar transferases | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACTTGCTCTGCCCCGCCTCTCCGCCCCAC |
| Internal bar code: | GCCAGTTGGTGGTTCGGAACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 786 |
| LEAP-Seq percent confirming: | 99.7113 |
| LEAP-Seq n confirming: | 4144 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGATCCAAAACAACGGCT |
| Suggested primer 2: | ACCAGTCGATAGGTCGGATG |