| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.045822 |
| Chromosome: | chromosome 7 |
| Location: | 4191688 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g342350 | PDE14 | (1 of 4) K13293 - cAMP-specific phosphodiesterase 4 (PDE4); 3'%252C5'-cyclic-nucleotide phosphodiesterase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCACGTATGCAGTACCTGCTCCTTGTCCA |
| Internal bar code: | GCTAGACACAGTGTGGCCTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 838 |
| LEAP-Seq percent confirming: | 91.7234 |
| LEAP-Seq n confirming: | 1751 |
| LEAP-Seq n nonconfirming: | 158 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACGCTATTGTCTGCCGTT |
| Suggested primer 2: | GTGTACACCAACGCCAACAG |