| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.045835 |
| Chromosome: | chromosome 9 |
| Location: | 4091840 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393543 | HCP2 | (1 of 4) 1.7.99.1 - Hydroxylamine reductase / Hydroxylamine (acceptor) reductase; Hybrid-cluster protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGAAGGAAGAACGCAACAGTAGTAGCAA |
| Internal bar code: | TGCGGTGGATCAGGTACTCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 540 |
| LEAP-Seq percent confirming: | 99.4671 |
| LEAP-Seq n confirming: | 3920 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACACATTCGGCACATAAC |
| Suggested primer 2: | CTTTGTTCCAGGTGCCATTT |