| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.045836 |
| Chromosome: | chromosome 15 |
| Location: | 946492 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g639200 | (1 of 8) 2.4.1.5 - Dextransucrase / Sucrose 6-glucosyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGCAGCTAGCCGTGGGACAGAAACAGCC |
| Internal bar code: | GACTGTGGCTCTCGTAAACGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 868 |
| LEAP-Seq percent confirming: | 94.7641 |
| LEAP-Seq n confirming: | 1828 |
| LEAP-Seq n nonconfirming: | 101 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGGTTGAGGAGATGCTG |
| Suggested primer 2: | AACTGAAACGAACCCAATGC |