| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.045852 |
| Chromosome: | chromosome 1 |
| Location: | 5508555 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g038850 | (1 of 1) PTHR24012//PTHR24012:SF308 - FAMILY NOT NAMED // PROTEIN Y55F3AM.3, ISOFORM A | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGGCGCTTCAATCGCCGCTGGATGTCCA |
| Internal bar code: | ACGTCCGACGGATTTTCCCCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 654 |
| LEAP-Seq percent confirming: | 98.9363 |
| LEAP-Seq n confirming: | 12185 |
| LEAP-Seq n nonconfirming: | 131 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAACTCCTCCTTCCACCT |
| Suggested primer 2: | CTGCTGAGCTCGAGTGACTG |