| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.045855 |
| Chromosome: | chromosome_1 |
| Location: | 2905592 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g017650 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGTGTGACGGATGCCTTGCGTGGACTCATC |
| Internal bar code: | GCCGTTCGCAGAATATGTTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 936 |
| LEAP-Seq percent confirming: | 92.4857 |
| LEAP-Seq n confirming: | 5674 |
| LEAP-Seq n nonconfirming: | 461 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGTGGCTGAGCTTGTATG |
| Suggested primer 2: | GCTTACAGGCCATGCTCTTC |