| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.045857 |
| Chromosome: | chromosome 17 |
| Location: | 6612179 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g743597 | MRPS107,mS107 | Mitochondrial ribosomal protein S107, Chlamydomonas specific | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGAATACATTTTAGGGCGTTTCTGTCAC |
| Internal bar code: | TGAACGAATGCAGTGCGCCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 644 |
| LEAP-Seq percent confirming: | 76.2012 |
| LEAP-Seq n confirming: | 4758 |
| LEAP-Seq n nonconfirming: | 1486 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGAAGGGGTGAATTAGAGC |
| Suggested primer 2: | CCGTGTCAGCAATCTCTCAA |