Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.045863 |
Chromosome: | chromosome 16 |
Location: | 969987 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre16.g648800 | (1 of 6) IPR000253//IPR008984 - Forkhead-associated (FHA) domain // SMAD/FHA domain | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACAAAGTCTTGCTGCAAGACGAACCTAG |
Internal bar code: | TACCACACGAACAGTAACCACA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 496 |
LEAP-Seq percent confirming: | 77.8635 |
LEAP-Seq n confirming: | 2624 |
LEAP-Seq n nonconfirming: | 746 |
LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TATGCTGATGCCGCTAGTTG |
Suggested primer 2: | CACGCAAGTATCCGCTACAA |