| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.045871 |
| Chromosome: | chromosome 10 |
| Location: | 5976312 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g462450 | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCTGATAACATGGCGCTAGGAGTTACGA |
| Internal bar code: | CATTTGGTGGAGTGGCCTCCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1137 |
| LEAP-Seq percent confirming: | 99.2712 |
| LEAP-Seq n confirming: | 5312 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTATGCGTTTTGGAAACT |
| Suggested primer 2: | TTGAGAGTGATGATGAGGCG |