| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.045901 |
| Chromosome: | chromosome 12 |
| Location: | 2276182 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g507872 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGGGCCGACAAGACCGCAGCAGCTTCTG |
| Internal bar code: | CACGCGTAATAGCCCGTTGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 202 |
| LEAP-Seq percent confirming: | 68.0952 |
| LEAP-Seq n confirming: | 143 |
| LEAP-Seq n nonconfirming: | 67 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCAGTTAATGTGGCAGCA |
| Suggested primer 2: | CGGCGAATACATCTGTTGGT |