Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.045936 |
Chromosome: | chromosome 14 |
Location: | 782260 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre14.g612950 | CYG17 | (1 of 70) 4.6.1.2 - Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTTTATAGATGAGGGATACCGTATTATT |
Internal bar code: | GTTTTGGGTGTGCGCCGGTGAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 914 |
LEAP-Seq percent confirming: | 99.2087 |
LEAP-Seq n confirming: | 5015 |
LEAP-Seq n nonconfirming: | 40 |
LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AATCCACAGTCCGACAGTCC |
Suggested primer 2: | GTTGCAGGGATTGAGGTTGT |