Insertion junction: LMJ.RY0402.045942_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.045942
Chromosome:chromosome 9
Location:4571623
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g396289 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAACGCCATCACCGCCGCCGCCCCGCCAC
Internal bar code:CCGCTCGTGGCGCGACTTTTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:710
LEAP-Seq percent confirming:76.3001
LEAP-Seq n confirming:2054
LEAP-Seq n nonconfirming:638
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGGATTGGCCTCATACAT
Suggested primer 2:TGCTGACGCTCATAAAGGTG