| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.045956 |
| Chromosome: | chromosome 6 |
| Location: | 2859468 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g271900 | (1 of 1) 1.1.99.37 - Methanol dehydrogenase (nicotinoprotein) / Nicotinoprotein methanol dehydrogenase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGACAACGCCCGCGGCGGCCGCAACGCGC |
| Internal bar code: | ATGGAATAACCGGACGCACAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 238 |
| LEAP-Seq percent confirming: | 99.4377 |
| LEAP-Seq n confirming: | 2299 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACCCCATTGTCCTGTCCA |
| Suggested primer 2: | TTCAACGACTTCGTGTCCAG |