| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.045958 |
| Chromosome: | chromosome 7 |
| Location: | 3657586 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g337250 | (1 of 110) 3.4.19.12 - Ubiquitinyl hydrolase 1 / Ubiquitin thiolesterase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCCGCCAAGCGGGTCTCACAGCTCCCAC |
| Internal bar code: | TTGTGCGTAGTGTATTTAGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 996 |
| LEAP-Seq percent confirming: | 99.6717 |
| LEAP-Seq n confirming: | 15482 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCATGTGGGTATTGCTTGT |
| Suggested primer 2: | CAAGCAAGGAGTCAGGAAGG |