| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.045991 |
| Chromosome: | chromosome 17 |
| Location: | 1752188 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g709000 | (1 of 1) PTHR11319//PTHR11319:SF35 - G PROTEIN-COUPLED RECEPTOR-RELATED // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCACCTGGTCCAGCGCATCTAGAAGCAA |
| Internal bar code: | GTCGAGAGGCCGGCTGGGCACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 891 |
| LEAP-Seq percent confirming: | 99.346 |
| LEAP-Seq n confirming: | 3038 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTGTAGAAAAGGGCGTTG |
| Suggested primer 2: | GCATCATCAACGTCAACTGG |