Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.046006 |
Chromosome: | chromosome 10 |
Location: | 261357 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g419500 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTATGAATGTAACCCCCCACCCTGCTGCG |
Internal bar code: | TCGCGGGCCGGGTTGGGAAATC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 500 |
LEAP-Seq percent confirming: | 99.5867 |
LEAP-Seq n confirming: | 13733 |
LEAP-Seq n nonconfirming: | 57 |
LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GACAGGATGACGTCAGGGTT |
Suggested primer 2: | GAAATGACCGTGCGTGTATG |