| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.046011 |
| Chromosome: | chromosome 3 |
| Location: | 7593386 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g204600 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGTGTATGGGGCTGTGCGCACGGTCACG |
| Internal bar code: | TAGGGGCACTGAGGGAGAAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 485 |
| LEAP-Seq percent confirming: | 98.3522 |
| LEAP-Seq n confirming: | 2089 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGGTCTGGTCGGTTCTGT |
| Suggested primer 2: | GCACTTACCTCTGGCTACGC |