| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.046013 |
| Chromosome: | chromosome 10 |
| Location: | 2576963 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g436950 | Putative nucleolar protein; (1 of 4) PF07534 - TLD (TLD) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTCACCTGTAAGGGCAAGGCACGTGTGT |
| Internal bar code: | CGGCGGCCGAGTCACTGCGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 464 |
| LEAP-Seq percent confirming: | 97.9518 |
| LEAP-Seq n confirming: | 2152 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGTGATTGGTGTGGGTTA |
| Suggested primer 2: | GTTCCTCCTTGTCTGCTTCG |