| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.046013 |
| Chromosome: | chromosome 12 |
| Location: | 1484244 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g487601 | (1 of 274) IPR020683 - Ankyrin repeat-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCCACCAGCTAGCCAGCCACCCCTGCCC |
| Internal bar code: | CGATCAGAATAGACAGGAGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1087 |
| LEAP-Seq percent confirming: | 99.3084 |
| LEAP-Seq n confirming: | 718 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTGACGGTGAGATTGTGG |
| Suggested primer 2: | GAGTGGAGTGAAGAGACGGC |