Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.046021 |
Chromosome: | chromosome 6 |
Location: | 8006470 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g304000 | SRH14 | (1 of 1) PTHR10799:SF580 - INTEGRATOR COMPLEX SUBUNIT 10-LIKE PROTEIN; SNF2-related DNA/RNA helicase | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGCGGGTGAGTTCGCAGCATGTGGCGTC |
Internal bar code: | TCGTGATCGGTAGAAAATCTTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 746 |
LEAP-Seq percent confirming: | 79.1599 |
LEAP-Seq n confirming: | 4843 |
LEAP-Seq n nonconfirming: | 1275 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGTTTTCTCCCAAACAAGCA |
Suggested primer 2: | ATGCGTGGGGTCTTAGTGTC |