Insertion junction: LMJ.RY0402.046021_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.046021
Chromosome:chromosome 6
Location:8006477
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g304000 SRH14 (1 of 1) PTHR10799:SF580 - INTEGRATOR COMPLEX SUBUNIT 10-LIKE PROTEIN; SNF2-related DNA/RNA helicase CDS/intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCGCTCTTGCCAGAGTGGTTGTACCGGA
Internal bar code:TTGTGGCGTGGTGGAGGACGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:359
LEAP-Seq percent confirming:58.6207
LEAP-Seq n confirming:85
LEAP-Seq n nonconfirming:60
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GGTTTTCTCCCAAACAAGCA
Suggested primer 2:ATGCGTGGGGTCTTAGTGTC