| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.046022 |
| Chromosome: | chromosome 17 |
| Location: | 1374435 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g706250 | (1 of 4) PTHR19959:SF128 - KINESIN LIGHT CHAIN 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGTCACACGTTTCTTCATCACTTAGCT |
| Internal bar code: | CCCTGGGAGCGTCCCCACCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 756 |
| LEAP-Seq percent confirming: | 97.9825 |
| LEAP-Seq n confirming: | 1117 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCATCAGAGTGTGCAAGC |
| Suggested primer 2: | GGTCACGTGAGATTGAGGGT |