Insertion junction: LMJ.RY0402.046046_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.046046
Chromosome:chromosome 14
Location:744872
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g612700 FAP50,DRC7 Nexin-dynein regulatory complex 7; (1 of 1) PF13415//PF13418//PF13855 - Galactose oxidase, central domain (Kelch_3) // Galactose oxidase, central domain (Kelch_4) // Leucine rich repeat (LRR_8) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCTCTCATCAACTCACCTTCACTGACGC
Internal bar code:CTGTAACGTGGAATGTCGTGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:919
LEAP-Seq percent confirming:98.8383
LEAP-Seq n confirming:9699
LEAP-Seq n nonconfirming:114
LEAP-Seq n unique pos:56

Suggested primers for confirmation by PCR

Suggested primer 1:GTAGCAACTCGCTTCCGAAC
Suggested primer 2:GGTCCATGGTCATCCAAAAC