| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.046046 |
| Chromosome: | chromosome_14 |
| Location: | 744897 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g612700 | DRC7,FAP50 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CAGCTCAGCCCCCCGTGTCCAGTCTGGGTC |
| Internal bar code: | TTGTGGCACTTCTATTTCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 253 |
| LEAP-Seq percent confirming: | 98.773 |
| LEAP-Seq n confirming: | 161 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGCAACTCGCTTCCGAAC |
| Suggested primer 2: | GGTCCATGGTCATCCAAAAC |