Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.046048 |
Chromosome: | chromosome_7 |
Location: | 2496370 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre07.g329500 | SUB1 | Secreted protease and protease inhibitor | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATGTGCACTCCTAGCATGGCCTACCTGAAA |
Internal bar code: | CATCCCAATCAGATAGGTCGTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 932 |
LEAP-Seq percent confirming: | 99.2768 |
LEAP-Seq n confirming: | 3981 |
LEAP-Seq n nonconfirming: | 29 |
LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTCTGTGAACAGGGCGGTAT |
Suggested primer 2: | ATTGCACCTGGAATGACACA |