Insertion junction: LMJ.RY0402.046066_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.046066
Chromosome:chromosome 6
Location:8987797
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g311650 IRK1 (1 of 1) PTHR11767//PTHR11767:SF58 - INWARD RECTIFIER POTASSIUM CHANNEL // FI16807P1; Inwardly-rectifying potassium channel CDS/intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAATGGTCTGCTGTGTCTCCGTGGCGTACA
Internal bar code:GTACTTCTCCTAACCCGTGCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:929
LEAP-Seq percent confirming:91.2673
LEAP-Seq n confirming:4494
LEAP-Seq n nonconfirming:430
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:AAGTGCGTGGGGTAGTTTTG
Suggested primer 2:GGGCTCAGGTCAATCCAGTA