| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.046068 |
| Chromosome: | chromosome 16 |
| Location: | 5383134 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g681500 | (1 of 1) K07002 - uncharacterized protein (K07002) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCCAAACCGTCTCGCTGTGACCCGTAGT |
| Internal bar code: | GCGGGAATTGCGACGGCAAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 778 |
| LEAP-Seq percent confirming: | 99.4382 |
| LEAP-Seq n confirming: | 885 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATGAAAGGGAGCCACTTA |
| Suggested primer 2: | CGGGTGTCTGTGTGGTAGTG |