| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.046078 |
| Chromosome: | chromosome 2 |
| Location: | 1633401 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g085279 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTACACTAGGGTTGTTGTCGGTTTTTTCG |
| Internal bar code: | TGGTCACTGCCGTCCCCAGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 525 |
| LEAP-Seq percent confirming: | 88.3929 |
| LEAP-Seq n confirming: | 1386 |
| LEAP-Seq n nonconfirming: | 182 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATGGAGAGAGTGTTCCCC |
| Suggested primer 2: | TTACATTTCATCCCACGGGT |