| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.046098 |
| Chromosome: | chromosome 17 |
| Location: | 3668050 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g726650 | CGL96 | (1 of 1) K14839 - nucleolar protein 16 (NOP16); Conserved in the Green Lineage | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCCTTACACGGTCGCGGCCGGCCGACTT |
| Internal bar code: | TCTCGCGGGAGACGGGCCCACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 42 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 142 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTGAGCACCTGACTCCT |
| Suggested primer 2: | TGCAGTACGAGCGGTATGAG |