Insertion junction: LMJ.RY0402.046103_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.046103
Chromosome:chromosome 6
Location:4071502
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g278213 LHCA6 Light-harvesting protein of photosystem I; (1 of 2) K08910 - light-harvesting complex I chlorophyll a/b binding protein 4 (LHCA4) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAGATTGCGATGGTGGGCCATCCCTGTTT
Internal bar code:CGCACGCTTGAAAACGTTTTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:504
LEAP-Seq percent confirming:99.5834
LEAP-Seq n confirming:5259
LEAP-Seq n nonconfirming:22
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TTTGACGCCTTGAAGCTTTT
Suggested primer 2:GGCTCGATTCTCTTCTCCCT