| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.046121 |
| Chromosome: | chromosome 1 |
| Location: | 3365283 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g021300 | (1 of 2) IPR016140 - Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCACCCGGCCTGCAGCAAGTAGACGAAC |
| Internal bar code: | TTCGCGTTGTGTCTAAATTACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1130 |
| LEAP-Seq percent confirming: | 99.443 |
| LEAP-Seq n confirming: | 37316 |
| LEAP-Seq n nonconfirming: | 209 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTATTGGCGTTGTGGTGA |
| Suggested primer 2: | GCACATATCCGTGCAATGAC |