Insertion junction: LMJ.RY0402.046161_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.046161
Chromosome:chromosome 14
Location:1200713
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g616000 (1 of 35) IPR001763 - Rhodanese-like domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGGGCACGGATTGAAACAATTACGAGCTC
Internal bar code:GTTGTCGGCGACTGGGCCTGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:710
LEAP-Seq percent confirming:99.7585
LEAP-Seq n confirming:3718
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTGATGCGACAAGCTCAT
Suggested primer 2:AAACCTGCAATACGTTTGGG