| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.046161 |
| Chromosome: | chromosome 14 |
| Location: | 1200776 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g616000 | (1 of 35) IPR001763 - Rhodanese-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGTCTGCTACTGGTGGGTAGGGGCAAGG |
| Internal bar code: | GTACGGGACGGAAAGCGGGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 610 |
| LEAP-Seq percent confirming: | 98.2469 |
| LEAP-Seq n confirming: | 4035 |
| LEAP-Seq n nonconfirming: | 72 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTGATGCGACAAGCTCAT |
| Suggested primer 2: | AAACCTGCAATACGTTTGGG |