Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.046182 |
Chromosome: | chromosome_3 |
Location: | 5432562 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g185050 | FAP53 | Flagellar Associated Protein | sense | CDS/intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGAGCTGGACCTGAAGATCCTGCAGGAGGC |
Internal bar code: | CGGTCGAGAGGCAGGGATCTCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 712 |
LEAP-Seq percent confirming: | 99.3117 |
LEAP-Seq n confirming: | 2453 |
LEAP-Seq n nonconfirming: | 17 |
LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTCTAATTTGCCCATCCCAG |
Suggested primer 2: | ATGGTACCCACAGTCGAAGC |