| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.046209 |
| Chromosome: | chromosome 10 |
| Location: | 1690769 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g430200 | ARS3 | Arylsulfatase; (1 of 19) 3.1.6.1 - Arylsulfatase / Sulfatase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGAGTTCCAAGGGTGCAGTCCCGAGAA |
| Internal bar code: | CCAATTGTCCTGATCCTGTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 171 |
| LEAP-Seq percent confirming: | 99.0991 |
| LEAP-Seq n confirming: | 110 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCATGGCCATTATGGATT |
| Suggested primer 2: | GCAGCAGCACAGGAATTACA |