Insertion junction: LMJ.RY0402.046210_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.046210
Chromosome:chromosome 6
Location:3469475
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g278096 SELU2,SELENOU,SELU1 Selenoprotein U2; (1 of 4) PF13911 - AhpC/TSA antioxidant enzyme (AhpC-TSA_2) 5'UTR_intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTAAACCCTGTCCTACTTCCTATCCACAGC
Internal bar code:CGAACCCGGCGCCTGGCGCGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1034
LEAP-Seq percent confirming:94.5098
LEAP-Seq n confirming:241
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TTGCGATCGATAAGGAAACC
Suggested primer 2:GCACATACTGATCACCACGG