Insertion junction: LMJ.RY0402.046238_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.046238
Chromosome:chromosome 1
Location:5188815
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g036100 (1 of 1) K11790 - denticleless (DTL, CDT2, DCAF2) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGTCACTCAGACGCAGCTGCAGCTGGCTG
Internal bar code:TGGTTTGCTACCCGGGATTTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:439
LEAP-Seq percent confirming:98.2819
LEAP-Seq n confirming:2517
LEAP-Seq n nonconfirming:44
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GCTCTTGTATCGACCGAAGC
Suggested primer 2:GTCAGAGTCGCAGTCCCAGT