| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.046271 |
| Chromosome: | chromosome 3 |
| Location: | 5899789 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g189950 | HOP1,HOP | HSP70-HSP90 organizing protein; (1 of 1) K09553 - stress-induced-phosphoprotein 1 (STIP1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAAGGCCGCGGATGCTGTAGTCCAGTGGC |
| Internal bar code: | CTCCCGATTATCGGGTAGGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 109 |
| LEAP-Seq percent confirming: | 99.6672 |
| LEAP-Seq n confirming: | 599 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGATGAGGGACGGATGAG |
| Suggested primer 2: | GTTCTCTTTGGCTCAGTGGC |