| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.046272 |
| Chromosome: | scaffold 24 |
| Location: | 90843 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre24.g755747 | (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCCACCATCTCCACCACGACCGCGTGCC |
| Internal bar code: | ACGTAACGTCGCGATCATGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 300 |
| LEAP-Seq percent confirming: | 84.3233 |
| LEAP-Seq n confirming: | 21677 |
| LEAP-Seq n nonconfirming: | 4030 |
| LEAP-Seq n unique pos: | 83 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGACCAGCAAAGGCAAGAA |
| Suggested primer 2: | GCTCCTCGTCCTCATACTGC |