Insertion junction: LMJ.RY0402.046287_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.046287
Chromosome:chromosome 3
Location:3579339
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g168250 (1 of 6) PF06701 - Mib_herc2 (MIB_HERC2) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCGGTCCGGTGCACTGCCTGACAGCCCA
Internal bar code:GCCGTTCTGGGGGCGGTCAGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:854
LEAP-Seq percent confirming:99.4745
LEAP-Seq n confirming:8329
LEAP-Seq n nonconfirming:44
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGAACTGGAGGTGAGAGG
Suggested primer 2:AGGGGCTAAGCGAGAGGTAG