| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.046292 |
| Chromosome: | chromosome 9 |
| Location: | 3866414 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391986 | (1 of 1) 3.1.1.32 - Phospholipase A(1) / Phospholipase A1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCAGCGACACTGCACAGCCGCAGCACGC |
| Internal bar code: | GCGGGTGGCCGATCTAGGTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 297 |
| LEAP-Seq percent confirming: | 53.4091 |
| LEAP-Seq n confirming: | 47 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGACGTTGTACTTGTCCCT |
| Suggested primer 2: | TCCAGCTAGGGCAACTGTCT |