Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.046292 |
Chromosome: | chromosome 11 |
Location: | 2097806 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCCGACCAGAGGGGCCGGAGGAACAAAC |
Internal bar code: | CGTGAGATGATAGCGAAGACGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 674 |
LEAP-Seq percent confirming: | 99.4586 |
LEAP-Seq n confirming: | 3858 |
LEAP-Seq n nonconfirming: | 21 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGTCGTACGGTTGACTGCTC |
Suggested primer 2: | CTGAAAGCGCCTTGTAGGAC |