Insertion junction: LMJ.RY0402.046365_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.046365
Chromosome:chromosome 12
Location:1823337
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g511950 (1 of 1) IPR003591//IPR013210 - Leucine-rich repeat, typical subtype // Leucine-rich repeat-containing N-terminal, plant-type intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGAAATGCGTCGCCCCCACGGTCAGCCAC
Internal bar code:CACGGACGGTCTACCAGGACCT

Confirmation - LEAP-Seq

LEAP-Seq distance:945
LEAP-Seq percent confirming:98.3319
LEAP-Seq n confirming:4480
LEAP-Seq n nonconfirming:76
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTACCCTTCCACATGCCT
Suggested primer 2:GCGGCAAAGGTGTAGAACAT