| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.046390 |
| Chromosome: | chromosome 10 |
| Location: | 1898598 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g431850 | PGM18 | Phosphoglycerate mutase family protein; (1 of 1) PTHR23029//PTHR23029:SF49 - PHOSPHOGLYCERATE MUTASE // PHOSPHOHISTIDINE PHOSPHATASE SIXA | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTCCCTCGCGGCCTCTGCCGCCGCATAT |
| Internal bar code: | GGTTTTGCTTGTTGTTTTTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 824 |
| LEAP-Seq percent confirming: | 93.7107 |
| LEAP-Seq n confirming: | 447 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGGCTCCAGACAACTTGC |
| Suggested primer 2: | CAAACACACAGGTTGCCAAG |