| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.046407 |
| Chromosome: | chromosome 12 |
| Location: | 600697 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g492500 | (1 of 39) IPR009003 - Peptidase S1, PA clan | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTACTCACTCCGGTCCTGCGCCTCCTGCGC |
| Internal bar code: | AAAAACCGAGCCTTTAAAGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 123 |
| LEAP-Seq percent confirming: | 1.26891 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 2023 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCAACATAGGACACCACG |
| Suggested primer 2: | CGGCAGCTTGTGTGTGTACT |