Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.046408 |
Chromosome: | chromosome_9 |
Location: | 3924275 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g392282 | DHC2 | Dynein heavy chain 2, inner arm dynein d (monomeric) | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ACAAGAAGCGCAGCACCTTGAACGGAATCA |
Internal bar code: | GGGTCATGTCGTCGGTCCGTCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1139 |
LEAP-Seq percent confirming: | 98.4848 |
LEAP-Seq n confirming: | 520 |
LEAP-Seq n nonconfirming: | 8 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCTACAAGGAGTCCAGTCCG |
Suggested primer 2: | AAGAAGCCGCTGATCCAGTA |