Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.046408 |
Chromosome: | chromosome 9 |
Location: | 3924292 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre09.g392282 | DHC2 | Axonemal Dynein Heavy Chain 2; (1 of 1) PTHR10676:SF137 - DYNEIN HEAVY CHAIN 1, AXONEMAL | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGCCGCGTGACGGACGACAAGGACCGC |
Internal bar code: | TAAGTCGTCTGCCAGGATCGCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 781 |
LEAP-Seq percent confirming: | 98.7826 |
LEAP-Seq n confirming: | 2840 |
LEAP-Seq n nonconfirming: | 35 |
LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCTACAAGGAGTCCAGTCCG |
Suggested primer 2: | AAGAAGCCGCTGATCCAGTA |