Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.046432 |
Chromosome: | chromosome_2 |
Location: | 4964645 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g103950 | FAP100 | Flagellar Associated Protein | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGGTAATGGAGACTATTGCAGACGCATGAC |
Internal bar code: | TGGGCCGAATGTCTTTGTAGGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 987 |
LEAP-Seq percent confirming: | 98.2241 |
LEAP-Seq n confirming: | 1604 |
LEAP-Seq n nonconfirming: | 29 |
LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTGAGGGTTGTGGATGTGTG |
Suggested primer 2: | CCAGGTACCCGTGCCTAGTA |