| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.046443 |
| Chromosome: | chromosome 12 |
| Location: | 5495584 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g530950 | TGL18 | (1 of 5) PF01734 - Patatin-like phospholipase (Patatin); Putative triacylglycerol lipase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCTTCAACGGGCTCGCGCCCAAGCACAG |
| Internal bar code: | GCAAAATGCAAAATACGAGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 716 |
| LEAP-Seq percent confirming: | 19.6653 |
| LEAP-Seq n confirming: | 47 |
| LEAP-Seq n nonconfirming: | 192 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACACACACACCCACACACA |
| Suggested primer 2: | ACTAGGGTGCTGGGACATTG |