Insertion junction: LMJ.RY0402.046473_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.046473
Chromosome:chromosome 2
Location:1927734
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g087850 PTK19 Protein tyrosine kinase; (1 of 2) 2.7.10.2//2.7.11.25 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase // Mitogen-activated protein kinase kinase kinase / MLTK CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGATCACCGTCTTCACCGCTACGGGAATG
Internal bar code:TCGCGGGAGGGGGCGCTAATAG

Confirmation - LEAP-Seq

LEAP-Seq distance:77
LEAP-Seq percent confirming:73.2394
LEAP-Seq n confirming:104
LEAP-Seq n nonconfirming:38
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CCTCCGTGAATGATACGCTT
Suggested primer 2:AAAACGGTGACGCATCTCTC